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Directory of Open Access Journals Sweden. Cultured hippocampus and cerebellar granule neurons from mutant animals were significantly smaller than neurons from wild type animals. In cultures from such doubly heterozygous female mice, the size of neurons expressing the mutant A V allele also showed a significant reduction compared to neurons expressing wild type MeCP2, supporting a cell-autonomous role for MeCP2 in neuronal development. IGF-1 insulin growth factor-1 treatment of neuronal cells from Mecp2 mutant mice rescued the soma size phenotype. Our results suggest that i reduced neuronal size is an important in vitro cellular phenotype of Mecp2 mutation in mice, and ii MeCP2 might play a critical role in the maintenance of neuronal structure by modulation of the mTOR pathway. Burn mouse models.
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Topical photodynamic therapy of squamous cell carcinomas in a hairless mouse model. In situ fluorescence measurement was used to monitor PpIX formation after the topical application of various concentrations of ALA cream to determine the optimal ALA dose. Cutaneous challenge with chemical warfare agents in the SKH-1 hairless mouse. I Development of a model for screening studies in skin decontamination and protection. Exposure to lethal chemical warfare agents CWAs is no longer only a military issue due to the terrorist threat.
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